This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Coller, J. A. Articles by Yin, L. K. Search for Related Content PubMed PubMed Citation Articles by Coller, J. A. Articles by Yin, L. K.

Radioimmunoelectrophoretic Binding Assay for the Detection of Carcinoembryonic Antigen¹

Hospital of the University of Pennsylvania, Department of Surgery and Harrison Department of Surgical Research, Philadelphia, Pennsylvania 19104

A new radioimmunoassay (RIA) method has been described. This radioimmunoelectrophoretic binding (REB) technique has been applied to the carcinoembryonic antigen system. In contrast to the RIA method with the use of perchloric acid extraction, in the REB technique the requirement for lengthy and possibly biologically degrading procedures on relatively large volumes of sera has been eliminated. Only one-five hundredth as much serum is required by REB than is required by RIA, and the assay can be completed in 2 hr rather than in 5 days. While the sensitivity of REB in the detection of serum carcinoembryonic antigen is similar, there appears to be a different but related specificity, compared to the RIA method.

¹ Funded in part by USPHS Training Grant GM 01540-06 and by Grant CI-18-3 from the American Cancer Society.

² Formerly a Clinical Fellow of the American Cancer Society. Present address: Department of Surgery, Lahey Clinic Foundation, 605 Commonwealth Avenue, Boston, Mass. 02215.

Received 12/27/72. Accepted 3/19/73.