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Stable Chromosome Changes in a Human Malignant Melanoma¹

Department of Biology, Graduate School of Biomedical Sciences, and M. D. Anderson Hospital and Tumor Institute, The University of Texas at Houston, Houston, Texas 77025

A cell culture was established from a human malignant melanoma. Karyotype analyses using some of the newer staining techniques revealed a modal chromosome number of 45 with the presence of a ring, a large marker chromosome with an interstitial C-band, and at least five other structural rearrangements resulting in marker chromosomes. Most of these changes occurred in vivo since they were identifiable in the biopsy material 4 hr after the culture was initiated. They persisted in early and later subcultures. Although the culture grew slowly, the ring remained relatively stable while endoreduplication decreased and 2S stem cells increased with time.

¹ Supported by USPHS Grant 1 P01 GM19513.

Received 3/ 9/73. Accepted 5/ 8/73.