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The Isolation and Characterization of Gallium-binding Granules from Soft Tissue Tumors¹

Medical Division, Oak Ridge Associated Universities,² Oak Ridge, Tennessee 37830

Both rate-zonal and isopycnic-zonal centrifugation experiments indicate that ⁶⁷Ga in rat tumors (RFT tumor and Morris 7777, 5123C, and 7794A hepatomas) and in a mouse lymphosarcoma (P-1798) is associated with lysosomal granules. The particles were identified enzymatically by their acid phosphatase and N-acetyl-ß-D-glucosaminidase content. Autoradiography of selected gallium-binding granule fractions showed silver grains concentrated over electrondense single-membrane organelles.

¹ This investigation was supported by USPHS Research Grant CA-11858 from the National Cancer Institute.

² Under contract with the U. S. Atomic Energy Commission.

Received 12/27/72. Accepted 5/15/73.