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Polyadenylate-containing RNA of Polyribosomes Isolated from Rat Liver and Morris Hepatoma 7800¹

McArdle Laboratory for Cancer Research, University of Wisconsin Medical School, Madison, Wisconsin 53706

The fraction of polyribosomal RNA that contains polyadenylate sequences was examined in rat liver and the Morris hepatoma 7800. Sequential sodium dodecyl sulfatephenol extraction at pH 7.6 and pH 9.0 followed by polythymidylate cellulose chromatography of the fraction extracted at pH 9.0 was used to isolate the polyadenylate-containing fraction of polyribosomal RNA. This fraction from either tissue appeared to be the same with respect to mobility on acrylamide gels, sensitivity to ribonuclease digestion, and ³²P base composition analysis. There was no difference in the absolute amount of polyadenylate-containing RNA in polyribosomes from either liver or hepatoma.

The time course of the decay in specific activity of the polyadenylate-containing RNA was determined following the administration of a single dose of ³²P. From these data, a half-life of approximately 9 to 12 hr could be calculated for the polyadenylate-containing RNA fraction from both liver and the hepatoma polyribosomes.

¹ The work described was supported in part by Grant CA-07175 from the National Cancer Institute and Grant E-588 from the American Cancer Society.

² Present address: Department of Chemistry, Biochemistry and Biophysics, Massey University, Palmerston North, New Zealand.

³ To whom correspondence should be addressed.

Received 7/16/73. Accepted 10/ 2/73.