This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Sharma, B. Articles by Terasaki, P. I. Search for Related Content PubMed PubMed Citation Articles by Sharma, B. Articles by Terasaki, P. I.

In Vitro Immunization to Cultured Human Tumor Cells¹

Department of Surgery, School of Medicine, University of California, Los Angeles, California 90024

Immunization of lymphocytes by 17 cultured human tumor lines and subsequent killing of the specific tumor target has been demonstrated. Killing, measured by the release of ⁵¹Cr from labeled target cells, was greater for the specific tumor lines in most instances than for the non-specific lines tested or for phytohemagglutinin-stimulated lymphocytes. In addition, lymphocytes immunized by human tumor cell lines were more reactive against the tumor lines than were lymphocytes immunized by allogeneic lymphocytes. Although immunization to HL-A antigens cannot be established as yet, simple immunization to tumor antigens cannot be excluded.

¹ This work was supported by Contract NIH-NCI 72-2008 within The Virus Cancer Program of the National Cancer Institute.

² To whom reprints requests should be addressed, at the Department of Surgery, School of Medicine, University of California, Los Angeles, Calif. 90024.

Received 8/10/73. Accepted 9/28/73.