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Strain-dependent Teratogenic Effects of 1-Ethyl-1-nitrosourea in Inbred Strains of Mice¹

The Jackson Laboratory, Bar Harbor, Maine 04609

Single i.p. injections of 1-ethyl-1-nitrosourea, 0.5 mmole/kg, were given to C57L/J, C57BL/6J, SWR/J, DBA/2J, and AKR/J mice on Days 8 and 12 of gestation, and fetuses were examined on Day 14, or after birth. High rates of fetal deaths were observed in C57L/J mice on Day 14; observations on earlier days revealed an increase in the resorption rate and a concomitant decrease in the percentage of malformations from days 10 to 14 of gestation, indicating that the early harmful effects of 1-ethyl-1-nitrosourea were incompatible with life.

The 1-ethyl-1-nitrosourea induced a variety of fetal malformations at both Day 8 and 12; the most common were of the brain and eyes, limbs, ribs, sternum, vertebrae, and ribs. The frequency and severity of these malformations varied with the stage of embryogenesis and the strain of mice. Strains C57L/J and C57BL/6J were most sensitive and strain AKR/J was most resistant; strains SWR/J and DBA/2J were intermediate.

¹ This study was supported in part by NIH Training Grant CA05013 from the National Cancer Institute to The Jackson Laboratory and by Research Contract NO1 CP 33255 within the Special Virus-Cancer Program of the National Cancer Institute. The Jackson Laboratory is fully accredited by the American Association for Accreditation of Laboratory Animal Care.

Received 6/15/73. Accepted 10/24/73.

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