This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gauvreau, D. Articles by de Lamirande, G. Search for Related Content PubMed PubMed Citation Articles by Gauvreau, D. Articles by de Lamirande, G.

Purification of a Ribonuclease Inhibitor from Novikoff Hepatoma Supernatant Fraction¹

Institut du Cancer de Montréal, Centre Hospitalier Notre-Dame, and Département de Biochimie, Université de Montréal, Montréal, Quebec, Canada

A comparison between the levels of ribonuclease inhibitor in normal rat liver and Novikoff hepatoma 105,000 x g supernatant fractions is reported. Novikoff hepatoma contained 61% less inhibitor per g of tissue or 39% less per mg protein than did normal rat liver.

A rapid and simple method that had been developed for the purification of rat liver ribonuclease inhibitor was applied to Novikoff hepatoma 105,000 x g supernatant fraction. The purification procedure led to a 121-fold increase in specific activity, with a yield of 49%. The molecular weight and inhibition curve of purified inhibitor were also determined and were found to be similar to those of normal rat liver ribonuclease inhibitor.

¹ This investigation was supported by grants from the National Cancer Institute of Canada, Le Ministère des Affaires Sociales du Québec, La Fondation J. H. Biermans, and Les Fondations J. Rhéaume.

² Recipient of a studentship from l'Institut du Cancer de Montréal.

³ Recipient of a studentship from the Medical Research Council of Canada.

⁴ Research Associate of the National Cancer Institute of Canada.

Received 3/18/74. Accepted 6/ 5/74.

This article has been cited by other articles:

				M. C. Haigis, E. L. Kurten, and R. T. Raines Ribonuclease inhibitor as an intracellular sentry Nucleic Acids Res., February 1, 2003; 31(3): 1024 - 1032. [Abstract] [Full Text] [PDF]