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Enhancement of Simian Virus 40-induced Transformation of Chinese Hamster Embryo Cells by 4-Nitroquinoline 1-Oxide¹

The Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104

Treatment of Chinese hamster embryo cells with 4-nitroquinoline 1-oxide (4NQO) enhanced the frequency of SV40-induced transformation. Treatment with 4NQO (0.4 µg/ml) for 2 hr at 2 or 7 hr preinfection produced a 2- to 10-fold increase in transformation, while treatment up to 8 hr postinfection enhanced transformation 2- to 3-fold. All cells transformed by 4NQO + SV40 or by SV40 alone contained SV40 T antigen. Although 4NQO induced cytotoxicity, enhancement of transformation was not due to selection of virus-susceptible, 4NQO-resistant cells present in the original population since the transformed cells showed no increase in resistance to the cytotoxicity. Cells transformed by 4NQO + SV40 had a lower growth potential during subsequent propagation than did cells transformed by SV40 alone.

¹ This investigation was supported in part by USPHS Grants CA 08936 and CA 10815 from the National Cancer Institute, HD 06323 from the National Institute for Child Health and Human Development, and RR 05540 from the Division of Research Resources; and by the Commonwealth of Pennsylvania.

² Present address: Department of Bacteriology, Tohoku University Medical School, Sendai, Japan.

Received 3/28/74. Accepted 6/28/74.