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A Peroxidase Inhibitor in Leukemic AKR Mouse Spleen Cells¹

Department of Pathology and Medical Research, St. Margaret's Hospital, and Department of Obstetrics and Gynecology, Tufts University School of Medicine, Boston, Massachusetts 02125

Spleen cell suspensions from leukemic AKR mice, unlike those from normal animals of the same strain, were found to be devoid of in vitro bactericidal activity. These cells were also found to be deficient in glucose 6-phosphate dehydrogenase, hexose monophosphate shunt, and peroxidase activities. In contrast, formate-¹⁴C oxidation, a measurement of H₂O₂, by spleen cells from leukemic animals was markedly elevated when compared to this activity of cells from nonleukemic AKR mice. The 20,000 x g pellet fraction of the spleen cell homogenates from leukemic animals was devoid of peroxidase activity. This fraction was not bactericidal in the presence of H₂O₂ and chloride ion at acidic pH. Indeed, this high speed pellet of homogenates of leukemic mice markedly inhibited peroxidase-mediated amino acid decarboxylation and bactericidal functions of the same fraction from homogenates of spleen cells from nonleukemic control animals. The inhibitory activity was found to be heat stable and nondialyzable.

¹ This investigation was supported by the United States Atomic Energy Commission Grant 3517-75 and NIH Grants Ca 5307 and HE 1805.

² Present address: Department of Microbiology, Albert Einstein Medical Center, Philadelphia, Pa. 19141.

Received 6/21/74. Accepted 8/16/74.