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Dietary Effects on Stearyl Coenzyme A Desaturase in Morris Hepatomas¹

Medical Division, Oak Ridge Associated Universities, Oak Ridge, Tennessee 37830

In the present study, we determined the effect of dietary changes on the activity of stearyl coenzyme A desaturase in two transplantable Morris hepatomas (5123C and 7777) and their host livers; the activity of fatty acid synthetase was also measured in the same preparations. Stearyl coenzyme A desaturase activities of the hepatomas were increased, whereas the fatty acid synthetase activities remained the same when tumor-bearing rats maintained on laboratory chow were fasted for 48 hr and refed for 48 hr. Similar results were obtained when rats were fed a fat-free diet for 2 months before transplanting the hepatomas and then continued on the same diet for 1 month (7777 tumors) or 2 months (5123C tumors). We conclude that acyl coenzyme A desaturase is regulated in hepatomas as it is in normal liver, and therefore it does not exhibit the lack of regulation that is characteristic of the enzymes that synthesize fatty acids and cholesterol in hepatomas.

¹ Supported by the United States Atomic Energy Commission and the American Cancer Society (BC-70E). A portion of this work was presented at the Meeting of the American Society of Biological Chemists in Minneapolis, Minn., 1974.

Received 5/ 2/74. Accepted 8/26/74.