This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Kuroda, Y. Search for Related Content PubMed PubMed Citation Articles by Kuroda, Y.

Effects of Hexosamines and Their Acetyl Derivatives on Aggregation of Rat Hepatoma Cells in Rotation Culture¹

Department of Morphological Genetics, National Institute of Genetics, Misima, Sizuoka 411, Japan

The effects of hexosamines and their acetyl derivatives on the aggregation of 4-dimethylaminoazobenzene-induced rat hepatoma cells, dRLa-74 and dRLh-84, which have a low and high tumor-producing activity, respectively, were examined in rotation-mediated cell culture.

D-Glucosamine, D-galactosamine, and D-mannosamine had relatively little effect on aggregation of either strain of hepatoma cells at concentrations lower than 3 mM. At concentrations higher than 10 mM, some differential inhibitory effects on the aggregation of the two hepatoma cell lines were observed. D-Glucosamine and D-galactosamine were more effective against the aggregation of dRLh-84 cells than in that of dRLa-74 cells, and the reverse was the case with D-mannosamine.

N-Acetyl-D-glucosamine, N-acetyl-D-galactosamine, and N-acetyl-D-mannosamine were relatively ineffective against the aggregation of dRLa-74 cells at all concentrations used. N-Acetyl-D-glucosamine had an inhibitory effect on the aggregation of dRLh-84 cells only at a concentration of 30 mM, while N-acetyl-D-galactosamine had a pronounced inhibitory effect at both 10 and 30 mM.

¹ This work was supported, in part, by a grant in aid for Fundamental Scientific Research from the Ministry of Education in Japan. Contribution No. 936 from the National Institute of Genetics, Misima, Japan.

Received 5/15/73. Accepted 10/ 1/73.