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Two-Dimensional Gel Electrophoresis of Acid-extractable Nuclear Proteins of Regenerating and Thioacetamide-treated Rat Liver, Morris 9618A Hepatoma, and Walker 256 Carcinosarcoma¹

Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77025

The acid-soluble nuclear proteins of regenerating and thioacetamide-treated rat livers as well as the Morris 9618A hepatoma and the Walker 256 carcinosarcoma were extracted from citric acid-isolated nuclei with 0.4 N H₂SO₄. The nuclear extracts were analyzed by two-dimensional polyacrylamide gel electrophoresis. Although most of the protein spots were common to the livers and tumors studied, all of the rodent tumors were similar in their marked density of Spots C16-C18. In normal, regenerating, and thioacetamide-treated liver, the spots in this region were much less dense.

¹ This project was supported by USPHS Grant CA-10893, P-3.

Received 8/ 2/73. Accepted 10/29/73.