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Differences in Distribution Pattern of Marker Enzymes among Subcellular Fractions from Morris Hepatoma 16¹

Department of Pathology, University of Missouri, Columbia, Missouri 65201 [P. J. C., M. L. V., F. V. L., J. A. E., A. P. M.], and the Department of Biochemistry, Cancer Research Unit, Howard University, Washington, D. C. 20001 [H. P. M.]

The distribution of marker enzymes in subcellular fractions isolated by differential centrifugation was determined in Morris hepatoma 16, host liver, and control rat liver. Marker enzymes of cellular organelles that were assayed included cytochrome oxidase, monoamine oxidase, ornithine aminotransferase, D-amino acid oxidase, acid phosphatase, glucose 6-phosphatase, ehtylmorphine demethylase, and lactic dehydrogenase. Comparison of host liver and hepatoma revealed significant differences in the distribution pattern of many of these enzymes, especially those associated with mitochondria. The hepatoma showed increased amounts of mitochondrial enzyme activity in a subcellular fraction requiring higher g forces than normal for sedimentation. This could mean that hepatoma 16 contains a greater proportion of mitochondria that are smaller in size and/or less dense than those of host or normal liver. Electron micrographs of hepatoma and host liver tissue and subcellular fractions also suggested that hepatoma 16 contains mitochondria that are smaller in size. It is concluded, therefore, that a greater proportion of mitochondria of hepatoma 16 are smaller in size than those of host liver.

¹ Presented in part at the 8th Midwest Regional Meeting of the American Chemical Society, Columbia, Mo., November 1972, p. 85, and the Fourth Bi-Annual Hepatoma Symposium, Howard University, Washington, D. C., May 1973. This work was supported in part by NIH Grants HL 12381 and CA 10729.

² This study is part of a dissertation submitted in partial fulfillment of the requirement for the degree of Doctor of Philosophy at the University of Missouri. Recipient of National Science Foundation Graduate Fellowship.

³ To whom requests for reprints should be addressed.

Received 1/ 2/73. Accepted 11/13/73.