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Induction of Hepatocarcinoma in Vivo with Fetal Mouse Liver Cells Spontaneously Transformed in Culture and Isolation of a Type C RNA Virus from the Carcinoma Cells¹

Microbiological Associates, Inc., Bethesda, Maryland 20014 [J. S. R., K. D. W., M. L. V.]; The Jackson Laboratory, Bar Harbor, Maine 04609 [H. W. C., H. M., C. W.]; and National Cancer Institute, Bethesda, Maryland 20014 [R. J. H.]

A hepatic cell line, FL83B/S2, derived from C57B/6J embryos, underwent spontaneous neoplastic transformation after long-term in vitro cultivation and produced progressively growing adenocarcinomas when inoculated into newborn isologous hosts. Transformation was accompanied by a conversion from murine leukemia virus group-specific antigen negativity to group-specific antigen positivity, and a type C RNA tumor virus was isolated from the tumors. This is the first report of type C virus isolation from murine hepatocarcinomatous cells.

¹ This work was supported by USPHS Contracts PH 43-70-2068 and NO1 CP 33255 within the Virus-Cancer Program of the National Cancer Institute.

Received 7/16/73. Accepted 11/19/73.