This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Lyser, K. M. Search for Related Content PubMed PubMed Citation Articles by Lyser, K. M.

Low- and High-Voltage Electron Microscopy of a Human Neuroblastoma in Long-Term Organ Culture

Institut d'Embryologie Expérimentale du Collège de France et du Centre National de la Recherche Scientifique, 49 bis, Avenue de la Belle-Gabrielle, 94130 Nogent-sur-Marne, France

A human neuroblastoma was maintained in a continuing organ culture, in which three-dimensional organization and cell proliferation occurred together. Electron micrographs of the cells showed a typical immature appearance: scant cytoplasm, numerous free ribosomes, poorly developed granular endoplasmic reticulum, and a large nucleus and prominent nucleolus. Cellular processes with microtubules were found to be present. Thus, the fine structure of the neuroblastoma in organotypic culture was similar to that of the original tumor and to ganglion cells at early stages of embryonic development.

¹ On sabbatical leave from the Department of Biological Sciences, Hunter College of the City University, New York, N. Y.

Received 7/13/73. Accepted 11/20/73.