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Thioacetamide-induced Alterations in Nuclear RNA Transport¹

Department of Pathology, University of Washington, Seattle, Washington 98195

Acute intoxication or prolonged feeding of male rats with thioacetamide is associated with an altered capacity of liver nuclei to transfer RNA to a surrogate cytoplasm in vitro. Control nuclei hydrolyze adenosine triphosphate and concomitantly release a 45 S ribonucleoprotein. Liver nuclei from animals receiving 5 or 20 mg of thioacetamide per 100 g body weight release a significant proportion of this ribonucleoprotein without adenosine triphosphate present. This ribonucleoprotein has identical sedimentation characteristics as that released in the presence of adenosine triphosphate. The enhanced energy-independent release appears not to be the result of altered nuclear fragility or altered composition of the nuclei. The response is time and temperature dependent. This system may provide an in vitro model for the analysis of carcinogen-related alterations in RNA metabolism during chemical carcinogenesis.

¹ These studies were supported in part by USPHS Grants CA-13600 and GM-13543.

Received 11/12/73. Accepted 1/11/74.