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The Cryopreservation of Colony-forming Cells from the Bone Marrow of Children with Acute Lymphocytic Leukemia¹

Division of Pediatric Oncology, The Edward Mallinckrodt Department of Pediatrics [A. H. R., E. G.], and the Division of Biostatistics [S. C. C.], Washington University, St. Louis, Missouri 63110

Bone marrow cells from 15 children with acute lymphocytic leukemia in remission were preserved in liquid nitrogen for periods of up to 16 weeks. Although a substantial reduction in the number of myeloid cells was noted after cryopreservation, the colony-forming cells were still capable of proliferation. Dimethyl sulfoxide was demonstrated to be superior to glycerol as a cryoprotective agent for human marrow cells.

¹ Supported in part by NCI Grant 5P02 CA 10435, the Children's Hematology Research Association of St. Louis, Mo., and American Cancer Society Grant CI-105.

² To whom requests for reprints should be addressed, at St. Louis Children's Hospital, 500 S. Kingshighway, St. Louis, Mo. 63110.

Received 11/ 5/73. Accepted 1/18/74.