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Oligonucleotides of Ribosomal 28 S RNA in Human Leukemic Cells and Normal Lymphocytes¹

Department of Medicine, Tumor Research Division, Universitatsklinikum, 43 Essen, Germany [S. S., K-P. B., J. K., C-G. S.], and Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77025 [H. B.]

Ribosomes were isolated and ribosomal RNA (rRNA) was prepared from leukemic cells derived from the venous blood of patients with acute myeloblastic leukemia, acute undifferentiated leukemia, subacute myelocytic leukemia, chronic lymphocytic leukemia, leukemic lymphosarcoma, leukemic reticuloendotheliosis, a cultured Burkitt lymphoma cell line, and phytohemagglutinin-stimulated normal lymphocytes. After in vitro labeling of the cells for 9 hr with orthophosphate-³²P, the 28 S rRNA was characterized by nucleotide analysis and by oligonucleotide frequency studies on dinucleotides and trinucleotides released by complete digestion with pancreatic RNase. Under identical labeling conditions, there was a significant difference in the specific activities of the 28 S rRNA of acute myeloblastic leukemia cells and phytohemagglutinin-stimulated lymphocytes. Following separations of the oligonucleotides of the RNase digests on DEAE-Sephadex A-25 at pH 7.6 according to chain length, minor variations were found in the oligonucleotide frequencies of 28 S rRNA within this group of leukemias; but there were no significant differences from the values for phytohemagglutinin-stimulated lymphocytes. Thus, in these cells, as in animal tumors, there is a remarkably constant composition and structural similarity of 28 S rRNA.

¹ These studies were supported by the Landesamt für Forschung, Nordrhein-Westfalen.

Received 9/21/73. Accepted 1/31/74.