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Effect of N-Methyl-N-nitrosourea on the Protein-synthesizing System in Mouse Liver and Hepatoma 22a Cells¹

Institute of Biological and Medical Chemistry, Academy of Medical Sciences, [O. Y. A., N. G. K., N. N. P., M. I. L.], Institute of Chemical Physics of the Academy of Sciences [L. B. G., I. S. S.], and Laboratory of Bioorganic Chemistry, Moscow University [T. Y. U.], Moscow, USSR

A single injection of nitrosomethylurea (80 mg/kg body weight) to mice bearing hepatoma 22a led to an injury of the main components of the protein-synthesizing system (polyribosomal complex and soluble factors of the cell sap) in liver and hepatoma cells.

In liver cells of adult animals, the activity of the protein-synthesizing system returns to a normal level 5 to 15 hr after injection, while in hepatoma the process is irreversible for at least 48 hr. These results were confirmed and extended in experiments conducted in vitro with cell-free protein-synthesizing systems.

¹ This work was supported by grants from the Academy of Medical Sciences and Academy of Sciences of the USSR.

Received 5/ 3/73. Accepted 2/ 1/74.