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6-Hydroxydopamine Sensitivity in Mouse Neuroblastoma and Neuroblastoma x L-Cell Hybrids¹

Department of Physiology and Cell Biology, University of Kansas, Lawrence, Kansas 66045 [R. L. C., P. E. T.]; Roswell Park Memorial Institute, Buffalo, New York 14203 [T. B. S.]; and Department of Biochemistry, Texas Technological University, School of Medicine, Lubbock, Texas 79409 [J. M.]

6-Hydroxydopamine sensitivity has been studied in mouse neuroblastoma cells, mouse L-cells, and in somatic cell hybrids between the two lines. Neuroblastoma cells are considerably more sensitive to this adrenergic nervous system antagonist than are the L-cells. This sensitivity is suppressed by 5-bromodeoxyuridine. Somatic cell hybrids initially showed the level of resistance of the L-cell parent, but 2 to 4 months after hybridization, two separate clonal isolates changed to the level of sensitivity of the neuroblastoma parent. Evidence suggests that this differentiated property is extinguished in somatic cell hybrids and reexpressed after chromosome loss.

¹ This work was supported by Grants 1 RO1 CA12310-01 and 5 RO1 HDO5196 from the USPHS. Portions of this investigation were presented at the meeting of the Genetics Society of America, August 1972 (9).

² Present address: Department of Entomology, Kansas State University, Manhattan, Kans. 66506.

Received 9/ 4/73. Accepted 3/22/74.