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Enhanced Na⁺-K⁺-activated Adenosine Triphosphatase Activity in Transformed Fibroblasts¹

Luka B. Kasrov and Herman Friedman

Department of Microbiology, Albert Einstein Medical Center, Philadelphia, Pennsylvania 19141

The activity of Na⁺-K⁺-activated adenosine triphosphatase was assayed in various lines of normal and transformed mouse and rat fibroblasts maintained in tissue culture. The rapidly growing, spontaneously transformed 3T6 and 3T12 cells derived from mouse 3T3 fibroblasts, as well as 3T3 cells transformed by SV40 and murine sarcoma-murine leukemia viruses and XC cells, a line established from a Wistar rat tumor induced with Rous sarcoma, showed a 4- to 5-fold greater activity than the normal nontransformed 3T3 Swiss and 3T3 BALB/c clone A31 cell lines. Of the total adenosine triphosphatase activity, 20 to 25% represented Na⁺-K⁺-activated adenosine triphosphatase in transformed cells, but only 5% in the normal cells. A hypothesis as to the role of Na⁺-K⁺-activated adenosine triphosphatase in controlling intracellular levels of cyclic adenosine 3',5'-monophosphate in transformed cells through competition for available substrate adenosine triphosphate was discussed.

¹ Supported in part by research grants from the National Science Foundation and the National Institute of Allergy and Infectious Diseases.

Received 10/11/73. Accepted 4/18/74.

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