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Modulation of Alkaline Phosphatase Activity in Cell Cultures Derived from Human Urinary Bladder Carcinoma

Department of Pathology, Sinai Hospital, Baltimore, Maryland 21215

T24 cells derived from a human urinary bladder carcinoma have very low alkaline phosphatase activity. The specific activity is 500 to 1000 times lower than that of HeLa S3 cells. Enzyme activity increases when T24 cells are grown in the presence of prednisolone (¹-hydrocortisone). Increase in the osmolality of the culture medium with NaCl or sucrose has a similar effect. When cells are grown in hyperosmolar medium with prednisolone added, a synergistic effect is noted. Neither stimulus significantly affects acid phosphatase activity.

¹ Present address: Department of Pathology, Montefiore Hospital and Medical Center, Albert Einstein College of Medicine, 111 E. 210th St., Bronx, N. Y. 10467

² Recipient of American Cancer Society-Eleanor Roosevelt-International Cancer Fellowship, awarded by the International Union Against Cancer, during the tenure of which fellowship this work was undertaken. Further support was provided by the Danish Research Council, The Rask Ørsted Foundation. The International Research Foundation of Denmark, Kobmand i Odense Johann og Hanne Weimann f. Seedorffs Legat, P. Carl Petersen Fond, and the Danish Foundation for the Advancement of Medical Science. Present address: Department of Pathology, Gentofte Hospital, University of Copenhagen, DK-2900, Hellerup, Denmark.

Received 1/31/74. Accepted 4/10/74.

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