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Incorporation of Arginine by Soluble Extracts of Ascites Tumor Cells and Regenerating Rat Liver¹

The Institute for Cancer Research, Fox Chase Center for Cancer and Medical Sciences, Philadelphia, Pennsylvania 19111

Soluble amino acid incorporation systems from ascites tumor cells, as well as from regenerating rat liver, incorporated arginine into the protein fraction in the absence of ribosomes. This incorporation is dependent on transfer RNA, adenosine triphosphate, and adenosine triphosphate generator, but not on guanosine triphosphate or an amino acid mixture. The reaction is inhibited by puromycin and RNase. The soluble amino acid incorporation system from the regenerated rat liver has 2 to 3 times higher activity for the incorporation of arginine than the system of the control. The soluble amino acid-incorporating system from ascites tumor shows 5 to 10 times higher incorporating activity than that from regenerated rat liver.

¹ This work was supported by NIH Grant CA-12572-02, National Science Foundation Grant GB-30252, and by grants to this Institute from NIH (CA-06927, RR-05539) and an appropriation from the Commonwealth of Pennsylvania.

Received 3/12/74. Accepted 5/15/74.