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Endogenous Type C Particles from Rat Embryo Cells Treated with 5-Bromodeoxyuridine¹

Departments of Pathology [S. A. S., S. P., E. S., W. H. K.] and Pediatrics [W. H. K.], Division of Biological Sciences, University of Chicago, Chicago, Illinois 60637

Type C particles were released from secondary W/Fu rat embryo cell cultures following exposure to 10^-4 M 5-bromodeoxyuridine for 36 hr. The particles were first detected in culture fluids by electron microscopy and uridine-³H labeling 2 days after drug treatment. Maximal release of type C particles occurred between 8 and 10 days. All rat embryo cells treated with 5-bromodeoxyuridine eventually fluoresced against antiserum to antigens from 2 mouse leukemia viruses. Fewer than 1% of the analog-treated cells contained budding particles as judged from electron microscopy. The induced type C particles banded at buoyant densities of 1.16 to 1.18 g/ml in sucrose equilibrium gradients. Culture fluids from 5-bromodeoxyuridine-treated rat embryo cells contained DNA polymerase activity. The type C particles failed to transform mouse or rat cells in vitro and did not induce neoplasms in neonatal mice or rats. 5-bromodeoxyuridine-treated rat embryo cells were not tumorigenic when injected into syngeneic newborns.

¹ This investigation was supported by USPHS Grant CA-14599 from the National Cancer Institute.

² Predoctoral trainee supported by NIH Training Grant HD-00297 from the National Institute of Child Health and Human Development.

³ Joseph P. Kennedy, Jr., Mental Retardation Research Center.

Received 3/14/74. Accepted 5/20/74.

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