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Specific Increase in Polyamine Levels in Chick Embryo Cells Transformed by Rous Sarcoma Virus

Department of Molecular Biology, The Hebrew University-Hadassah Medical School, Jerusalem, Israel

Chick embryo fibroblasts and chorioallantoic membranes of chick embryos infected with oncogenic or nononcogenic viruses were analyzed for polyamines. Nononcogenic viruses (influenze, Newcastle disease, or vaccinia virus) had no effect on the polyamine content of chorioallantoic membranes. Transformation of chorioallantoic membranes by a wild-type or temperature-sensitive mutant strain of Rous sarcoma virus under permissive conditions (37°) caused a 2- to 4-fold increase in cellular spermidine and putrescine content. Only putrescine accumulated in chick embryo fibroblasts transformed by Rous sarcoma virus at 37°. At the nonpermissive temperature (42°), the temperature-sensitive mutant, unlike the wild-type strain, did not alter cellular morphology or polyamine content.

Received 8/ 2/74. Accepted 8/26/74.