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RNA Sulfurtransferase Activity in Rat Liver and Chemically Induced Hepatomas¹

Department of Biochemistry, West Virginia University School of Medicine, Morgantown, West Virginia 26506

RNA sulfurtransferase activity has been detected in rat liver and in hepatomas from rats fed a diet containing 0.06% 3'-methyl-4-dimethylaminoazobenzene for 14 to 18 weeks. The reaction measured was the transfer of sulfur from cysteine to acceptor sites in Escherichia coli B transfer RNA (tRNA). Specific activities of the enzymes in liver and hepatoma supernatant fractions were similar, as were the rates and extents of sulfur transfer to tRNA. DEAE-cellulose chromatography of digests of the [³⁵S]tRNA reaction products revealed 3 peaks associated with nucleotide material, the amounts of these peaks differing in tRNA from liver and hepatoma systems. This may suggest differences in specific sulfurtransferases in these tissues.

¹ This work was supported in part by USPHS Grant CA-16567-01 from the National Cancer Institute.

Received 6/ 5/75. Accepted 8/22/75.