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Cytogenetic, Cytological, and Virological Characteristics of a Bovine Fibrosarcoma¹

Section of Viral Oncology, Comparative Leukemia Studies Unit [C. A. D., R. R. M., J. F. F.], Laboratory of Anatomy [W. C. D. H.], and Laboratory of Pathobiology [D. C. D.], School of Veterinary Medicine, University of Pennsylvania, New Bolton Center, Kennett Square, Pennsylvania 19348

The features of a bovine tumor with typical histopathological characteristics of a malignant fibrosarcoma are described. Direct karyotype preparations from the tumor tissue showed two populations of cells with bizarre karyotypes. An in vitro cell culture, designated BS-2, derived from the tumor had a normal diploid karyotype. This culture formed syncytia and produced a virus morphologically indistinguishable from the ubiquitous bovine syncytial virus. BS-2 cells reacted strongly in immunofluorescent tests with both bovine syncytial virus reference serum and serum from the tumorous cow. The virus was also demonstrated by immunofluorescence and mixed culture techniques in the buffy coat cells of the same cow.

¹ This work was supported in part by USPHS Cancer Grant 1-PO1-CA-14193-02, Pennsylvania Department of Agriculture Grant ME-4, and United States Department of Agriculture Cooperative Agreement 12-14-100-10,675(45).

² Postdoctoral fellow on USPHS Grant 5-TO1-CA-05097-13. To whom reprint requests should be addressed.

³ Present address: Animal Disease Research Institute, Canada, Department of Agriculture, Health of Animals Branch, 801 Fallowfield Road, P. O. Box 11300, Postal Station H, Ottowa, Ontario, K2H 8P9, Canada.

Received 7/ 9/75. Accepted 9/ 9/75.