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The Role of Serum Factors in the Acceleration by Freund's Complete Adjuvant of the Growth of Transplanted Murine Leukemic Cells¹

Department of Pediatrics, UCLA School of Medicine, Harbor General Hospital Campus,² Torrance, California 90509 [P. E. B., J. Z. F., K. L. T., C. H., D. T. I.], and Department of Microbiology and Immunology, UCLA School of Medicine, Los Angeles, California 90024 [P. E. B., D. T. I.]

Attempted nonspecific immunotherapy led to acceleration rather than retardation of tumor growth. Mice given injections of Freund's adjuvant were more susceptible to transplanted syngeneic Gross virus-induced leukemic cells when Freund's complete adjuvant was administered i.p. 0 to 7 days before or 1 day after tumor; thereafter, the adjuvant had no effect.

Two serum-mediated phenomena were demonstrated in vitro: (a) sera from mice immunized with Freund's complete adjuvant and tumor facilitated killing of tumor cells by peritoneal exudate cells from nonimmune mice; (b) sera from all mice with progressive tumor blocked the cytotoxicity of a xenogeneic tumor-specific serum.

Certain sera produced both effects. However, sera that either blocked or facilitated tumor killing in vitro had no effect on the growth in vivo of transplanted tumor cells.

¹ Supported in part by Cancer Research Funds of the University of California; The California Institute for Cancer Research; American Cancer Society, California Division Special Grant 568; National American Cancer Society Grant E-451B; National Cancer Institute Grant CA 08930; and the Mark Richardson Memorial Fund. Results included here were presented in part at the 15th Annual Meeting of the American Society of Hematology, Hollywood, Fla., December 1972.

² Address reprint requests here.

Received 4/13/73. Accepted 11/ 5/74.