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Transplantability of L1210 Cell in BALB/c x DBA/2F₁ Mice Associated with Cell Agglutinability by Concanavalin A¹

Division of Experimental Chemotherapy, Cancer Chemotherapy Center, Japanese Foundation for Cancer Research, Kami-Ikebukuro, 1-37-1, Toshima-ku, Tokyo 170, Japan

L1210 cells treated with different concentrations of glutaraldehyde at ice-cold temperature were characterized from various aspects. With 0.013% glutaraldehyde, the cells lost transplantability in BALB/c x DBA/2F₁ mice, and, concomitantly, lost cell agglutinability as measured by concanavalin A. They were, however, able to exclude trypan blue, and their cell surfaces were similar to those of the intact cells in susceptibility to mechanical disruption and in absorption of cytotoxic activity of rabbit anti-L1210 cell serum. Precise analysis indicated that transplantability was closely associated with cell agglutinability but not with excludability of trypan blue. On the basis of these results, the experimental condition was suggested in which immunization could be done safely to induce resistance against L1210 leukemia.

¹ This research was supported in part by a Grant-in-Aid for Cancer Research 1974 from the Ministry of Education of Japan.

Received 6/11/74. Accepted 10/16/74.