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Biochemical Characterization of Putative Subviral Particulates from Human Malignant Breast Tumors¹

Meloy Laboratories, Inc., Springfield, Virginia 22151 [R. M.]; Institute of Cancer Research, College of Physicians and Surgeons, Columbia University, New York, New York 10032 [S. S.]; and National Cancer Institute, NIH, Bethesda, Maryland 20014 [J. S.]

Particulates with the properties of cores and/or ribonucleoproteins of RNA tumor viruses have been isolated from Sterox-SL-treated fractions of murine and human mammary adenocarcinomas. These particulates have an RNA-directed DNA polymerase, a 60 to 70 S RNA, and a density of 1.26 g/ml or greater in sucrose equilibrium density gradients. Their uniquely higher densities lead to banding in regions comparatively free of cellular contaminants. These circumstances minimize some of the technical complications of performing the simultaneous detection assay in the presence of cell debris.

¹ This research was supported by Contracts NIH-NCI-CP-33258 and NIH-NCI-CA04223 within the Virus Cancer Program of the National Cancer Institute.

Received 9/20/74. Accepted 12/16/74.

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