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Specific Depression of the Antitumor Cellular Immune Response with Autologous Tumor Homogenate

Cell Biology Section, Viral Biology Branch, National Cancer Institute, NIH, Bethesda, Maryland 20014

A homogenate of an SV40-transformed fibrosarcoma of BALB/c mice (E₄ tumor) injected i.p. into E₄ tumor-immune syngeneic mice specifically depressed their cell-mediated immune responses to autologous tumor cells, as measured by a radioisotopic foot pad assay. The fraction of the tumor homogenate that brought about this depression was present in the high-speed supernatant and pellet of a 3 M KCl extract of the tumor. The specificity of the depression was shown in three ways: (a) the serum of E₄ tumor-immune mice, but not of normal mice, given injections of E₄ tumor homogenate 24 hr previously, suppressed antitumor immunity in vitro, as measured by the release of ⁵¹Cr from labeled E₄ tumor cells incubated with spleen cells from tumor-immune animals; (b) the i.p. inoculation of E₄ tumor homogenate did not alter the cellular immune response of tuberculin-sensitized mice to tuberculin; and (c) the i.p. injection of a homogenate of antigenically unrelated tumor did not depress the cellular immune response of E₄ tumor-immune mice to E₄ tumor cells.

Received 10/23/74. Accepted 2/ 5/75.