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Stability and Dissociation of P3HR-1 Burkitt's Lymphoma Cell Soluble Complement-fixing Antigen Identified with Human Serum

Chemical Sciences Department, Systems Group of TRW, Inc., Redondo Beach, California 90278

The soluble complement-fixing antigen of the P3HR-1 Burkitt's lymphoma cell line, identified with human serum containing antibody against Epstein-Barr virus viral capsid antigen, loses activity under a variety of conditions. The major characteristic reported here is retention of activity on exposure to specific physical or chemical conditions followed by loss of activity after subsequent neutral dialysis. Antigen of untreated cell lysates, which retains activity after dialysis against large volumes of neutral buffers or 0.14 M NaCl, will lose activity if the lysate is first heated to 56° or if the lysate is exposed to acid perchlorate, and the resulting precipitate and supernatant are redialyzed separately to neutral pH. The P3HR-1 soluble complement-fixing antigen appears to be an aggregate including a labile, dissociable component.

¹ This work was conducted under Contracts NIH-NCI-7200 and N01-CP-3-3252 within the Virus Cancer Program of the National Cancer Institute, NIH, USPHS.

Received 10/16/74. Accepted 3/11/75.