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Kinase and Deaminase Activity in a Variety of Subcutaneous Mouse Tumors¹

Biochemical Pharmacology Division, Kettering-Meyer Laboratories, Southern Research Institute, Birmingham, Alabama 35205

Extracts of solid mouse tumors were examined for deoxycytidine kinase and deaminase activities. 1-ß-D-Arabinofuranosylcytosine nucleotide was formed at a rate of 45 nmoles/hr by Glioma 26/57 and only 14 nmoles/hr by Ridgway osteogenic sarcoma. Deaminase activity was highest in Lewis lung (114 nmoles of 1-ß-D-arabinofuranosyluridine formed per hr) and in CaD₂ (104 nmoles of 1-ß-D-arabinofuranosyluridine formed per hr). Deaminase activity in tumor extracts is sensitive to freezing, while deaminase activity in monkey serum is not. It was observed that kinase activity varies by as much as 50% in different cell lines of the same tumor. In the presence of tetrahydrouridine, kinase activity was significantly increased in most of the tumors studied.

¹ Supported by Contracts NO1-CM-50654 and NO1-CM-43780 with the Division of Cancer Treatment, National Cancer Institute, NIH, Department of Health, Education, and Welfare. A preliminary report of this work has appeared (4).

Received 9/ 5/74. Accepted 3/25/75.