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The Production of Acidic Polysaccharides by 5-Bromodeoxyuridine-treated B16 Mouse Melanoma Cells¹

Departments of Biological Chemistry [C. S., J. B., S. G., E. A. D.] and Pathology [J. W. K.], The Milton S. Hershey Medical Center, The Pennsylvania State University, Hershey, Pennsylvania 17033

B16 melanoma cells were treated in culture with 5-bromo-2-deoxyuridine. The cell-associated and released proteoglycans and sialoglycopeptides were compared to those of control cultures treated with thymidine. The 5-bromo-2-deoxyuridine-treated cultures showed a marked reduction in the proportion of cell-associated proteoglycans and sialoglycopeptides, an increase in the synthesis of hyaluronic acid, the absence of high-molecular-weight chondroitin sulfate, and the presence of increased amounts of heparan sulfate in the media. In addition, the 5-bromo-2-deoxyuridine-treated cells had a higher DNA content and were larger than controls.

¹ This work was supported in part by USPHS Grants CA15483 and CA11097 and by USPHS Contract 70-2024.

² Career Development Awardee, 7-K4-CA38,809.

³ To whom all correspondence should be addressed.

Received 3/24/75. Accepted 5/14/75.