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Effect of Adriamycin on the Cell Cycle Traverse and Kinetics of Cultured Human Lymphoblasts¹

Laboratory of Cytokinetics, Sidney Farber Cancer Center and Harvard Medical School, Boston, Massachusetts 02115

Exposure of cultured human lymphoblasts to adriamycin (ADM) (0.1 µg/ml for 24 hr or 0.5 µg/ml for 1 hr) leads to an accumulation of cells with the DNA content of late S and G₂. Higher concentrations of ADM (0.5 to 10 µg/ml) inhibit cell cycle traverse. Effect of ADM on cell cycle traverse, cell growth, and incorporation of labeled precursors into DNA is dependent on drug concentration and length of exposure to ADM. Synchronized cells in G₁ or G₂ part of the cell cycle are less sensitive to ADM than cells in S phase. Similarly, plateau-phase cells are less sensitive to ADM than cells from log-phase cultures.

¹ Supported by Grant CA-06516 from the National Cancer Institute.

² The abbreviations used are: ADM, adriamycin; CHO, Chinese hamster ovary; TdR, thymidine; IMPY, 2,3-dihydro-1H-imidazo[1,2-b]pyrazole; Ll, Labeling index expressed as percentage of cells showing incorporation of labeled thymidine.

Received 5/27/75. Accepted 8/20/75.