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Establishment of a Cell Line (Co-115) from a Human Colon Carcinoma Transplanted into Nude Mice¹

Unit of Human Cancer Immunology, Lausanne Branch, Ludwig Institute for Cancer Research [S. C.]; Department of Immunology, Swiss Institute for Experimental Cancer Research [B. S.]; and Department of Biochemistry, University of Lausanne [C. M.], 1011 Lausanne Switzerland

A human colon carcinoma cell line, Co-115, has been established in vitro from solid xenografts maintained in nude mice and subcultured for 95 passages. Co-115 cells grow in vitro as tightly packed, epithelial-like colonies, have a doubling time of about 36 hr, have a relatively low plating efficiency in agar, and release significant amounts of carcinoembryonic antigen to the culture medium. Their epithelial nature has been confirmed by ultrastructural examination. The injection of Co-115 cells into nude mice reinduced the formation of solid tumor masses that could be retransplanted and showed a morphology comparable to that of the original xenograft.

¹ Supported in part by Grants 3.500.75 and 0841.4200 from the Swiss National Foundation for Scientific Research.

² To whom requests for reprint should be addressed, at Ludwig Institute for Cancer Research, 1066 Épalinges s/Lausanne, Switzerland.

Received 5/17/76. Accepted 8/ 9/76.