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Identification and Isolation of the Major Core Protein from the Oncornavirus-like Particle in Human Milk¹

Department of Biology, Michigan Cancer Foundation, Detroit, Michigan 48201

Subviral cores have been prepared from the oncornavirus-like particle found in human milks with the use of phospholipase C and ether or Sterox SL. The major protein of these cores has a molecular weight of 27,000 daltons, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. This protein is found in the core fractions of reverse transcriptase-positive milks and is absent in negative milks. It is distributed in sucrose gradients only in those fractions containing cores and reverse transcriptase activity. The major core protein of the human milk oncornaviruslike particle is electrophoretically identical to the major core protein of the mouse mammary tumor virus.

¹ This work was supported by Contract NOI-CP-33347 within the Virus Cancer Program of the National Cancer Institute, Grant CA 14100 from the National Cancer Institute, and an institutional grant from the United Foundation of Detroit.

² To whom requests for reprints should be addressed.

Received 4/ 9/76. Accepted 8/ 9/76.