This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Isohashi, F. Articles by Sakamoto, Y. Search for Related Content PubMed PubMed Citation Articles by Isohashi, F. Articles by Sakamoto, Y.

Specific Receptors for Glucocorticoid in the Cytoplasm of the Liver of AH 130 Tumor-bearing Rats¹

Department of Biochemistry, Institute for Cancer Research, Osaka University Medical School, Fukushima-1-chome, Fukushima-ku, Osaka, 553, Japan

Specific receptors for dexamethasone (11ß,17,21-trihydroxy-9-fluoro-16-methyl-1,4-pregnadiene-3,20-dione) in the cytoplasm of the liver from AH 130 (solid type) tumor-bearing rats markedly increased in the advanced stage of tumor growth. The cytoplasmic receptors of the livers of normal and tumor-bearing rats differed in their affinities for dexamethasone, and their apparent equilibrium (dissociation) constants (K) for dexamethasone were 4.0 and 2.6 x 10^-9 M, respectively. The rates of dissociation of dexamethasone-receptor complexes and the heat denaturations of the receptors in the livers of normal and tumor-bearing rats were similar. The glucocorticoid receptors of tumor-bearing rat liver had slightly higher affinities than did those of normal liver for all the steroids tested. Only a trace amount of receptors for dexamethasone could be detected in the cytoplasm of AH 130 ascites cells.

¹ This work was presented at the 34th Annual Meeting of the Japanese Cancer Association (1975), Osaka, Japan, and Annual Symposium of the Institute for Protein Research (1976), Osaka, Japan. It was supported by grants from the Ministry of Education, Science and Culture in Japan; the Osaka Anticancer Association; and Byotai Taisha Kenkyu Kai, Tokyo, Japan.

Received 6/ 3/76. Accepted 8/27/76.