This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Lozzio, C. B. Articles by Bamberger, E. G. Search for Related Content PubMed PubMed Citation Articles by Lozzio, C. B. Articles by Bamberger, E. G.

Absence of Thymus-derived Lymphocyte Markers in Myelogenous Leukemia (Ph¹+) Cell Line K-562¹

University of Tennessee Memorial Research Center, Center for Health Sciences, 1924 Alcoa Highway, Knoxville 37920 [C. B. L., B. B. L., A. T. I., E. G. B.], and Biology Division, Oak Ridge National Laboratory, Oak Ridge [W-K. Y.], Tennessee 37820

The myelogenous leukemia cell line K-562 with a Ph¹+ chromosome, derived from a patient with chronic myelogenous leukemia in terminal blastic crisis, is not a bone marrow-derived lymphoblastic cell line, because the cells neither produce immunoglobulins nor possess complement receptors. Since it has been suspected that blasts found in some patients with chronic myelogenous leukemia in blastic crisis might be thymus-derived cells, we have studied several parameters to demonstrate that K-562 cells are not thymus-derived lymphoblasts. The results of this study show: (a) no cross-reactivity of antisera to K-562 cells with normal human thymocytes; (b) lack of cytotoxicity of a specific horse anti-human thymocyte globulin for K-562 cells; (c) failure of the treatment of K-562 cells with bovine thymosin to induce antigenic determinant and erythrocyte rosette receptors on K-562 cells; (d) presence of receptors for the Fc portion of immunoglobulin G; (e) absence of terminal deoxynucleotidyl transferase; and (f) cytotoxicity of monkey antiserum to K-562 cells for malignant thymus-derived cells (Molt-4). However, absorption with Molt-4 cells abolished the cross-reactivity with Molt-4 cells, whereas 60% of the antibody to K-562 cells remained in the immune serum. Studies of DNA polymerase activities revealed that K-562 cells have levels of polymerase and ß, like other proliferating cells, and an RNA-dependent DNA polymerase activity, presumably representing polymerase .

¹ Aided by American Cancer Society Grants CI 87C and CH 5D and jointly by the United States Energy Research and Development Administration and the National Cancer Institute (VCP 20208) under contract with Union Carbide Corporation.

Received 6/23/76. Accepted 9/13/76.