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Presence of a High-Molecular-Weight RNA and RNA-directed DNA Polymerase in Rabbit Hereditary Lymphosarcoma¹

The Jackson Laboratory, Bar Harbor, Maine 04609

Rabbit lymphosarcoma tissues contain 70 S RNA and RNA-directed DNA polymerase encapsulated in particulate components that band in the density region of type C RNA viruses. RNA-directed DNA polymerase associated with the particles could be distinguished from cellular DNA polymerases by salt elution from phosphocellulose. The enzyme preferred the template primers poly(rA)·(dT)_12–18 and poly(rC)·(dG)_12–18 over other synthetic template primers and also utilized viral 70 S RNA as template; these properties are not observed with the known cellular DNA polymerases.

¹ This work was supported in part by NIH NRSA Fellowship CA05084; a grant from the Council for Tobacco Research, USA, NIH Research Grant RR-00251 from the Division of Research Facilities and Resources; and USPHS Research Contract N01 CP 33255 within the Special Virus Cancer Program of the National Cancer Institute.

Received 6/10/76. Accepted 9/14/76.

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