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Characteristics of a Surface-adherent Subline Derived from Friend Erythroleukemia Cells in Continuous Suspension Culture

Division of Laboratories and Research, New York State Department of Health, Albany, New York 12201

A surface-adherent cell population developed spontaneously from Friend erythroleukemia cells (FLC745) which previously had grown continuously in suspension cultures. The adherent cells have been transferred through more than 60 passages and apparently represent a stable variant, herein designated FLC745-Ad. The cell size, chromosome complement, and tumorigenicity in DBA/2 mice were similar to the parental FLC745 line, although the FLC745-Ad cultures grew at a slightly slower rate and synthesized less hemoglobin in response to dimethyl sulfoxide. Production of C-type particles was abundant in both suspension and adherent cells, but the latter appeared to contain more intracisternal A-type particles. Scanning electron microscopy disclosed that most adherent cells were globoid and nonspreading. They attached to the substrate by a circumferential lamellar skirt of unusual breadth. Adhesion of the FLC745-Ad cells was not influenced by serum concentration, trypsinization, 5-bromodeoxyuridine, or N⁶,O^2'-dibutyryl adenosine 3':5'-monophosphate. Treatment with cytochalasin B increased the cell size and the area of cell attachment.

¹ Present address: Sloan-Kettering Institute for Cancer Research, 145 Boston Post Road, Rye, N. Y. 10580.

² To whom requests for reprints should be addressed.

Received 6/18/75. Accepted 11/ 3/75.