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Methylation Analysis of the Carbohydrate Portion of Carcinoembryonic Antigen¹

Department of Immunology, City of Hope National Medical Center, Duarte, California 91010

The carbohydrate structural units of carcinoembryonic antigen samples isolated from four different tumors were quantitated using gas chromatography-mass spectrometery after methylation and subsequent conversion to their alditol acetates. Different carcinoembryonic antigen preparations showed some quantitative but no qualitative differences in the structural units present. The results indicate that a large portion of the fucose residues in the glycoprotein were linked to N-acetylglucosamine and that most of the branching mannose residues were probably linked to three N-acetylglucosamine residues.

¹ This research was supported by Grants CA 12631 and CA 16434, and by Contract NO1-CB-23877 from the National Cancer Institute, and by the Stuart L. Bernath Cancer Research Fund.

² To whom requests for reprints should be addressed, at Department of Immunology, City of Hope National Medical Center, Duarte, Calif. 91010. Present address: Rockefeller University, New York, N. Y. 10021.

Received 11/17/75. Accepted 2/18/76.