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Influence of Glucocorticoid, Estrogen, and Androgen Hormones on Transformation of Human Cells in Vitro by Feline Sarcoma Virus¹

Department of Veterinary Pathobiology, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio 43210

Infection of human foreskin cells (D-550) by the Snyder-Theilen strain of feline sarcoma virus produced small but countable foci and demonstrated "single-hit" dose-response kinetics. Significant quantitative and qualitative enhancement of focus formation was observed when the glucocorticoid hormones, dexamethasone, hydrocortisone, cortisol acetate, and prednisolone were added to cell cultures (1.0 µg/ml) 24 hr postinfection. However, aldosterone, while inducing qualitatively larger foci, did not bring about a quantitative enhancement in total foci number. By contrast, 17ß-estradiol, progesterone, cortisone acetate, methyltestosterone, and estrone elicited little or no effect on focus induction by Snyder-Theilen feline sarcoma virus. Evidence is suggestive of a posttranscriptional effect possibly modulating viral genome expression resulting in an increased efficiency of viral transformation, and an increased proliferation of transformed cells.

¹ Supported in part by Contracts CP-43217 and WO1-CP53571 from the Virus Cancer Program of the National Cancer Institute and by Contracts NIH-NCI-72-2047 and NO1-CP-43276 from the National Cancer Institute.

Received 9/22/75. Accepted 2/25/76.