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Growth Support of Small B16 Melanoma Implants with Nitrosourea-sterilized Fractions of the Same Tumor¹

Kettering-Meyer Laboratories, Southern Research Institute, Birmingham, Alabama 35205

B16 melanoma cells sterilized in vitro with 1,3-bis(2-chloroethyl)-1-nitrosourea or in vivo with trans-1-(2-chloroethyl)-3-(4-methylcyclohexyl)-1-nitrosourea, have been used to enhance the percentage of tumor takes with small s.c. implants of viable cells and to reduce the latent period between tumor implantation and palpability. The admixture of trans-1-(2-chloroethyl)-3-(4-methylcyclohexyl)-1-nitrosourea-inactivated cells with viable cell implants reduced the number of cells required to produce tumors in 50% of the animals by approximately 3 log₁₀ units and markedly reduced the time of tumor appearance from implants of up to 10⁶ cells. Similar results were obtained with 1,3-bis(2-chloroethyl)-1-nitrosourea-sterilized cells. The growth-supporting effect obtained with the nitrosourea-inactivated cells appeared to be as pronounced as that previously reported, for this tumor system, with radiation-inactivated cells.

¹ Supported by Contract NO1-CM-12098 from the Division of Cancer Treatment, National Cancer Institute, NIH, Department of Health, Education, and Welfare.

² To whom requests for reprints should be addressed.

Received 12/16/75. Accepted 2/27/76.