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In Vitro Malignant Transformation of Mouse Fibroblasts by Non-K-Region Dihydrodiols Derived from 7-Methylbenz(a)anthracene, 7,12-Dimethylbenz(a)anthracene, and Benzo(a)pyrene¹

Memorial Sloan Kettering Cancer Center, New York, New York 10021 [H. M.], and Chester Beatty Research Institute, Institute of Cancer Research: Royal Cancer Hospital, Fulham Road, London SW3 6JB, England [P. L. G., P. S.]

The 8,9-dihydrodiols of 7-methylbenz(a)anthracene and 7,12-dimethylbenz(a)anthracene and the 7,8-dihydrodiol of benzo(a)pyrene, which are non-K-region diols with adjacent olefinic double bonds that can be metabolized to diol-epoxides, were more active than the parent hydrocarbons in inducing malignant transformation of M2 mouse fibroblasts; a fourth non-K-region diol, the 9,10-dihydrodiol of benzo(a)pyrene was less active than benzo(a)pyrene.

The related K-region dihydrodiols, which lack adjacent olefinic double bonds, and 6-hydroxybenzo(a)pyrene were inactive. 7,8-Dihydrobenzo(a)pyrene, a more potent carcinogen than the 9,10 isomer, induced malignant transformation, but the 9,10 isomer was inactive. Transformed cells with abnormal morphology yielded sarcomas on injection into isologous mice; treated but morphologically normal cells did not. These results support the role of diols and diol-epoxides in the metabolic activation of polycyclic hydrocarbons.

¹ Supported, in part, by Grants CA08748 and CA15205 from the National Cancer Institute, USPHS, and by grants to the Chester Beatty Research Institute from the Cancer Research Campaign and the Medical Research Council. A preliminary report of some of these results has appeared (17).

² Recipient of Research Career Development Award 1KO4 CA00127, NIH, USPHS. To whom requests for reprints should be addressed.

Received 12/19/75. Accepted 3/ 3/76.

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