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Presence of Blood Group H Antigen on a Carcinoembryonic Antigen, and Its Enzymatic Modification into Blood Group A and B Specificities¹

Equipe de Recherche du Centre National de la Recherche Scientifique no. 62, Ecole Nationale Supérieure de Chimie, Montpellier [R. M., C. L., M. M-C.], and the Laboratoire de Biochimie II, Faculté de Pharmacie de Montpellier, Montpellier, France [J. P. B.]

⁴ Director of Research, Centre National de la Recherche Scientifique. To whom requests for reprints should be sent, at Ecole National Supérieure de Chimie, Montpellier, 8, rue de l'Ecole Normale, 34075 Montpellier Cedex, France.

A carcinoembryonic antigen (CEA-M) was purified from a hepatic metastasis obtained from a blood group O patient with cancer of the rectum. Using ¹²⁵I-labeled carcinoembryonic antigen (CEA) and blood group antisera, H specificity has been found on the CEA-M. As the addition of anti-H to anti-CEA does not modify the extent of binding of labeled CEA-M to its antibodies (86%), the H and CEA determinants are carried by the same molecule. The affinity chromatography of CEA-M on an immunosorbent "anti-H-Sepharose" demonstrated that a proportion of CEA-M molecules might bear both H and CEA antigenic determinants. In addition, glycosyltransferases were used to modify the blood group H specificity into blood group A or B specificities.

¹ This work was supported by the Centre National de la Recherche Scientifique, the Institut National de la Santé et de la Recherche Medicale, and la Fondation pour la Recherche Medicale Française.

² Research Fellow, Laboratoire de Biochimie II, Faculté de Pharmacie Montpellier.

³ Research Fellow, the Centre National de la Recherche Scientifique, Ecole National Supérieure de Chimie, Montpellier, France.

Received 7/ 7/75. Accepted 1/21/76.