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Karyotypes of Vasoformative Sarcomas Arising from BALB/3T3 Cells Attached to Polycarbonate Plates¹

Chromosome Research Unit, Faculty of Science, Hokkaido University, Sapporo, Japan[M. C. Y., M. S.], and Cell Biology Section, Laboratory of Carcinogenesis, National Cancer Institute, NIH, Bethesda, Maryland 20014 [N. T., C. W. B.]

Four vasoformative sarcoma in vivo tumor lines arising from the s.c. implantation of 3 x 10⁴ BALB/3T3 cells attached to a 1- x 5- x 10-mm polycarbonate platelet were shown to be quite similar in chromosome constitution to the parental 3T3 line. All tumors contained the same marker chromosomes that were present in the parent BALB/3T3 cells. The findings conclusively showed that the tumors had derived from BALB/3T3 cells and not from host cells or from the in vivo fusion of the inoculated 3T3 cells with host cells. Each tumor line had a unique karyotype that was markedly more homogeneous than that of the parental BALB/3T3 cells, strongly suggesting that each tumor represented a separate clone. An M1 marker chromosome was consistently present in each of the four tumor lines but was present in only about 50% of the parent BALB/3T3 cells; it therefore appeared to be a distinct and stable feature of the tumors.

¹ Supported in part by grants for Cancer Research from the Ministry of Education, Science and Culture, Japan.

Received 2/ 5/76. Accepted 3/30/76.