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A Galactosidase Immunosorbent Test for Carcinoembryonic Antigen¹

Laboratory of Experimental Immunology and Biophysics, The Miriam Hospital-Brown University, Providence, Rhode Island 02906

A galactosidase immunosorbent test for carcinoembryonic antigen (CEA) is described in which the amount of galactosidase adsorbed to a cellulose disc is a hyperbolic function of CEA concentration. Thus, molecules with CEA-like activity can be characterized by mathematical analysis of data obtained from the galactosidase immunosorbent test. By such analysis, CEA-reactive molecules in normal human plasma were distinguished from normal cross-reacting antigen and from authentic CEA. Variation of the amount of antibody-enzyme conjugate used in the galactosidase immunosorbent test permitted CEA-reactive material in plasma of a patient with rectal carcinoma to be antigenically distinguished from the CEA-reactive material in urine of a patient with bladder carcinoma. The galactosidase immunosorbent test is a useful tool for analysis of CEA-reactive molecules.

¹ This investigation was supported by USPHS Grant CA-15261 from the National Cancer Institute through the National Large Bowel Cancer Project.

² Submitted in partial fulfillment of the requirements for the Ph.D. degree at Brown University.

³ To whom requests for reprints should be addressed.

Received 11/11/75. Accepted 4/30/76.