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In Vitro Correlates of Transformation in C3H/10T Clone 8 Mouse Cells¹

Division of Hematology-Oncology, Department of Medicine, Children's Hospital, Los Angeles, California, 90027 [P. A. J., W. E. L., A. G., C. A. N., W. F. B.], and Department of Pathology, Colorado Medical Center, Denver, Colorado [L. M. F.]

Various potential in vitro correlates of malignancy were studied in four chemically transformed C3H/10T Clone 8 mouse cell lines and were compared with control cells. The degree of tumorigenicity was best predicted by the relative plating efficiencies of the morphologically transformed cells in soft agar. All transformed cells also showed an increase in extracellular fibrinolytic activity which may be an additional marker for transformation. Intracellular fibrinolytic activity and loss of ¹²⁵I-labeled cell surface protein (M.W. 250,000) were not correlated with morphological transformation or tumorigenicity in these cells.

¹ This work was supported in part by Grants CA-14226 and CA-15109 and Contract NO1 CP 55611 from the National Cancer Institute, NIH, and a grant from the American Cancer Society.

² Present address: Department of Medical Biochemistry, Box 63, Tygerberg 7505, Republic of South Africa.

³ Recipient of Career Development Award CA-00050 from the NIH.

⁴ Recipient of Career Development Award CA-70996 from the NIH. To whom requests for reprints should be sent.

Received 2/24/76. Accepted 4/26/76.

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