This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Hentzen, D. Search for Related Content PubMed PubMed Citation Articles by Hentzen, D.

Analysis of the Transfer RNA Population of Mouse Mammary Glands Infected with a Latent Mammary Tumor Virus¹

Electro-Radiologie, U.E.R. Médicale, Université Louis Pasteur, Strasbourg, France

Mammary gland transfer RNA's (tRNA's) of C3H mice infected with mammary tumor virus were analyzed in the preneoplastic state and compared to tRNA's of virus-free C3Hf mice and another uninfected strain, C57BL/6, which is completely resistant to cancer.

This quantitative study was based on the ability of each tRNA to fix its corresponding amino acid. The amount of each of the 17 tRNA's tested was identical for the three mammary glands. In addition, tRNA populations during lactation correlated with the amino acids incorporated into the lactoproteins synthesized, which indicates adaptation of the tRNA's to protein biosynthesis.

Qualitative chromatographic studies on reverse phase capillary columns Type 5 of 10 aminoacyl-tRNA's did not reveal any difference in the isoacceptor elution profiles. This shows that no new isoaccepting tRNA is associated with the mammary tumor virus at that stage, and that no viral modification of a host tRNA has occurred.

¹ This work was supported by the Ligue Nationale Française contre le Cancer.

² Present address: Département de Biologie générale et appliquée, Université Cl.Bernard, 43, Bd du 11 Novembre 1918, 69621 Villeurbanne, France.

Received 12/ 4/74. Accepted 5/25/76.